biography and scientific activity

BIOGRAPHY

Dr.Ammendola (Naples, 1959), in 1987 received the master’s degree, with a thesis on Zinc-finger proteins, in Physic at the University Federico II of Naples, Faculty of Mathematics, Physics and Natural Sciences. In 1991 He moved at Menarini Ricerche Sud (Pomezia-IT) as biotechnologist working on heterologous expression of enzymes of pharmaceutical interest. In 1999 He moved at Bioprogress SpA (Anagni-IT) at the head of biotechnology working at new processes to enzyme assisted synthesis of antiinflammatory drugs. In 2003 he received the PhD in Physiology and clinic of the skeletal system  at Sapienza University of Rome, Faculty of Medicine. In 2011, as owner of Ambiotec sas (Roma) he worked on production of food supplements. In 2015 he received the degree in Pharmaceutical applied sciences at Sapienza University of Rome, Faculty of Farmacy and Medicine. In 2018 he received a master’s degree in Human nutrition sciences at the University of Tor Vergata- Faculty of Medicine and Surgery. In 2022 he foudend Nanotechpharma SRL (Pomezia-IT) to develop nanoparticles of Active Pharmaceutical Ingredients.

MAIN PUBBLICATION

Ammendola S, Scotto d’Abusco A. Nutraceuticals and the Network of Obesity Modulators. Nutrients. 2022 Dec 1;14(23):5099. doi: 10.3390/nu14235099. PMID: 36501129; PMCID: PMC9739360.                                – Mariano A, Bigioni I, Mattioli R, Di Sotto A, Leopizzi M, Garzoli S, Mariani PF, Dalla Vedova P, Ammendola S, Scotto d’Abusco A. Harpagophytum procumbens Root Extract Mediates Anti-Inflammatory Effects in Osteoarthritis Synoviocytes through CB2 Activation. Pharmaceuticals (Basel). 2022 Apr 9;15(4):457. doi: 10.3390/ph15040457. PMID: 35455454; PMCID: PMC9026917.                                          – Mariano A, Di Sotto A, Leopizzi M, Garzoli S, Di Maio V, Gullì M, Dalla Vedova P, Ammendola S, Scotto d’Abusco A. Antiarthritic Effects of a Root Extract from Harpagophytum procumbens DC: Novel Insights into the Molecular Mechanisms and Possible Bioactive Phytochemicals. Nutrients. 2020 Aug 23;12(9):2545. doi: 10.3390/nu12092545. PMID: 32842461; PMCID: PMC7551290.                                                                -Ammendola S, Scotto d’Abusco A. Oxidative stress, senescence Mediterranean diet effects on osteoarthritis. in Aging: Oxidative Stress and Dietary Antioxidants. Academic Press 2020 Cap7: 73-80.                                — Ammendola S, Cocchiola R, Lopreiato M, Politi L, Scandurra R, Giusti AM, Scotto d’Abusco A. Olive Fruit Blends Modulate Lipid-Sensing Nuclear Receptor PPARγ, Cell Survival, and Oxidative Stress Response in Human Osteoblast Cells. J Am Coll Nutr. 2018 Sep-Oct;37(7):589-597. doi: 10.1080/07315724.2018.1451409. Epub 2018 Apr 13. PMID: 29652575.                                                        – – Ammendola S, Cocchiola R, Lopreiato M, Scotto d’Abusco A. Effects of Nutrients, Mainly from Mediterranean Dietary Foods, on Mesenchymal Stem Derived Cells: Growth or Differentiation. Int. J. Cli. Nut. Diet. 2018; 4:131-142 doi.org/10.15344/2456-8171/2018/131                                                                      -Ammendola S, Loreto MD, d’Abusco AS. Modulatory Effects of a Nutraceutical Supplement on Saos-2 Cells Reveal Its Phlebotonic Activity. J Am Coll Nutr. 2017 May-Jun;36(4):268-272. doi: 10.1080/07315724.2016.1269622. Epub 2017 Apr 26. PMID: 28443720.                                                            -Ammendola S, Stoppoloni D, Loreto MD, d’Abusco AS. A Nutraceutical Composition Decreases CPK Levels in Saos-2 Cells and in Patients with Elevated Serum Levels of This Enzyme. J Am Coll Nutr. 2016 Aug;35(6):559-567. doi: 10.1080/07315724.2015.1093972. Epub 2016 Jun 17. PMID: 27314492.                    -Cilia E, Teso S, Ammendola S, Lenaerts T, Passerini A. Predicting virus mutations through statistical relational learning. BMC Bioinformatics. 2014 Sep 19;15(1):309. doi: 10.1186/1471-2105-15-309. PMID: 25238967; PMCID: PMC4261881.                                                                                                                        -Ammendola S, Giusti, A M, Masci, A, Mosca, L, Saso, L, Bovicelli, P. Antioxidant properties of hydroxytyrosyl acetate compared with hydroxytyrosol and their protective capacity against oxidative stress in human neuroblastoma cells. Jour. Sci. Ind. Res. 2011; 70(11):929-937- ISSN 0022-4456.                                  -Cilia E, Sergio A. Identification of the amino acid residues affecting the catalytic pocket of the Sulfolobus solfataricus signature amidase. Protein Pept Lett. 2010 Feb;17(2):146-50. doi: 10.2174/092986610790226021. PMID: 20214638.                                                                                              -Radogna F, Paternoster L, De Nicola M, Cerella C, Ammendola S, Bedini A, Tarzia G, Aquilano K, Ciriolo M, Ghibelli L. Rapid and transient stimulation of intracellular reactive oxygen species by melatonin in normal and tumor leukocytes. Toxicol Appl Pharmacol. 2009 Aug 15;239(1):37-45. doi: 10.1016/j.taap.2009.05.012. Epub 2009 May 19. PMID: 19463840.                                                                    -Giordano C, Ammendola S. Characterization of mutants of Sulfolobus solfataricus signature amidase able to hydrolyse R-ketoprofen amide. Protein Pept Lett. 2008;15(6):617-23. doi: 10.2174/092986608784966949. PMID: 18680459.                                                                                                                                                  -Ammendola S, Mosca L, Bovicelli P. An expedient synthesis of 2,5-dihydroxytyrosol and studies on its effects on cell growth inhibition. ARKIVOC 2008; 8:105-115. dx.doi.org/10.3998/ark.5550190.0009.810 13-Paolo Bovicelli, Roberto Antonioletti, Silvia Mancini, Stefano Causio, Giorgio Borioni, Sergio Ammendola & Maurizio Barontini (2007) Expedient Synthesis of Hydroxytyrosol and its Esters, Synthetic Communications, 37:23, 4245-4252, DOI: 10.1080/00397910701575509                                                            -Cerella C, Mearelli C, Ammendola S, De Nicola M, D’Alessio M, Magrini A, Bergamaschi A, Ghibelli L. Molecular determinants involved in the increase of damage-induced apoptosis and delay of secondary necrosis due to inhibition of mono(ADP-ribosyl)ation. Ann N Y Acad Sci. 2006 Dec;1090:50-8. doi: 10.1196/annals.1378.005. PMID: 17384246.                                                                                                        -Sagratini G, Fabbri A, Marucci G, Ricciutelli M, Vittori S, Ammendola S. HPLC–MS validation of QualisaFoo® biosensor kit for cost-effective control of acrylamide levels in Italian coffee. Food Control 2007;18(10):1267-1271. doi.org/10.1016/j.foodcont.2006.08.005.
-Cilia E, Fabbri A, Uriani M, Scialdone GG, Ammendola S. The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles. Ser195 and Cys145 are predicted to be the active site nucleophiles. FEBS J. 2005 Sep; 272(18):4716-24. doi: 10.1111/j.1742-4658.2005.04887.x. PMID: 16156792.                                                                                                                 -Gianni W, Ricci A, Gazzaniga P, Brama M, Pietropaolo M, Votano S, Patanè F, Aglianò AM, Spera G, Marigliano V, Ammendola S, Agnusdei D, Migliaccio S, Scandurra R. Raloxifene modulates interleukin-6 and tumor necrosis factor-alpha synthesis in vivo: results from a pilot clinical study. J Clin Endocrinol Metab. 2004 Dec;89(12):6097-9. doi: 10.1210/jc.2004-0795. PMID: 15579764.                                                            -Scotto d’Abusco A, Ammendola S, Scandurra R, Politi L. Molecular and biochemical characterization of the recombinant amidase from hyperthermophilic archaeon Sulfolobus solfataricus. Extremophiles. 2001 Jun;5(3):183-92. doi: 10.1007/s007920100190. PMID: 11453462.                                                                      -Hoa NT, Baccigalupi L, Huxham A, Smertenko A, Van PH, Ammendola S, Ricca E, Cutting AS. Characterization of Bacillus species used for oral bacteriotherapy and bacterioprophylaxis of gastrointestinal disorders. Appl Environ Microbiol. 2000 Dec;66(12):5241-7. doi: 10.1128/aem.66.12.5241-5247.2000. PMID: 11097897; PMCID: PMC92451.                                                                                                               -Ammendola S, Politi L, Scandurra R. Cloning and sequencing of ISC1041 from the archaeon Sulfolobus solfataricus MT-4, a new member of the IS30 family of insertion elements. FEBS Lett. 1998 May 29;428(3):217-23. doi: 10.1016/s0014-5793(98)00530-4. PMID: 9654137.                                                         -Ammendola S, Raucci G, Incani O, Mele A, Tramontano A, Wallace A. Replacing the glutamate ligand in the structural zinc site of Sulfolobus solfataricus alcohol dehydrogenase with a cysteine decreases thermostability. Protein Eng. 1995 Jan;8(1):31-7. doi: 10.1093/protein/8.1.31. PMID: 7770449. 22-Ammendola S, Raia CA, Caruso C, Camardella L, D’Auria S, De Rosa M, Rossi M. Thermostable NAD(+)-dependent alcohol dehydrogenase from Sulfolobus solfataricus: gene and protein sequence determination and relationship to other alcohol dehydrogenases. Biochemistry. 1992 Dec 15;31(49):12514-23. doi: 10.1021/bi00164a031. PMID: 1463738.